Table 4.: Barcodes as an Indication of Phenotypic Diversity Among Isolates of E. coli Recovered from Different Surface Waters

Barcodea Number of Isolates Per Barcode (% of 45 Total) Isolate and Surface Water that Yielded Observed Barcode (Phenotype)b
Pond Stream/creek Lake River
5144572-36560 11(24.4) SHP-1
BKP
SRC
CTC-1
DL
IGL
FLN
RPL
DPR
ER-1
LWR
5144552-36560 7 (15.6) UHP
EP
AP
FAS
KC
RL ER-2
4144572-36570 4 (8.9) CTC-2
CTC-3
LH
LS
5144572-36570 3(6.7) LP-1
LP-2
WP
5144572-26570 3(6.7) CTPP FP CTC-4
5144572-26560 2(4.4) KFCP SVNCS
5144552-26570 2(4.4) TP ER-3
5044552-34560 2(4.4) AHP LM
5044572-34570 2(4.4) HP CP
5144562-36560 1(2.2) MRM
5144552-26560 1(2.2) TPS
5144472-36560 1(2.2) WPP
5044552-34570 1(2.2) JP
5044552-24560 1(2.2) LC
5044542-34760 1(2.2) CTC-5
4144572-26560 1(2.2) SHP-2
1044552-30560 1(2.2) BYC
0044552-30570 1(2.2) MRI
5144552-36560 E. coli ATCC 25922

a Per barcode, the first set of numbers (7 digits) represented the ID code generated for an isolate by the API 20E system, and the second set of numbers (5 digits) represented the ID code generated for the same isolate by the Enterotube II system. These were combined to form a 12-digit barcode. All codes identified isolates as E. coli.
b Use isolate abbreviation and Table 1 to determine the source of the isolate (i.e., name and location of the surface water sampled).