Table 1.: Bacterial strains and plasmids.

Strain Genotype Reference
V. fischeri
ES114 Wild-Type [42]
KV6576 IG (yeiR-glmS)::lacIq This Study
KV7432 IG (yeiR-glmS)::lacIq IG (VF_A0340-VF_A0341)::Tn5P (Ermr) This Study
KV7433 IG (yeiR-glmS)::lacIq cheZ::Tn5P (Ermr) This Study
Plasmid Description Reference
pCA24N lacIq (Cmr) [43]
pCLD46 LacI-repressible RscS overexpression plasmid (Cmr) [13]
pEVS104 Conjugal helper plasmid (Kanr) [32]
pEVS107 Mini-Tn7 delivery plasmid (Kanr Ermr) [15]
pEVS170 Tn5 carrier plasmid (Kanr Ermr) [31]
pJET1.2 Commercial cloning vector (Ampr) Fermentas
pJMO8 pKV363 containing the yeiR-glmS IG region (Cmr) This Study
pJMO10 pEVS170 containing a promoter within the Tn (Tn5P) (Kanr Ermr) This Study
pJMO14 pJMO8 containing the lacIq allele (Cmr) This Study
pKV363 Mobilizable suicide vector (Cmr) [22]
pVSV105 Mobilizable vector (Cmr) [12]