Acetate kinase Activity and Kinetic Properties of the Enzyme in Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 Intestinal Bacterial Strains

Table 1.: Acetate kinase activity in different fractions obtained from the bacterial cells.

Sulfate-reducing bacteria	Specific activity of the enzyme (U × mg-1 protein)
	Cell-free extract	Individual fractions
	Cell-free extract	Soluble	Sedimentary
Desulfovibriopiger Vib-7	1.52 ± 0.163	1.14 ± 0.118	0
Desulfomicrobium sp. Rod-9	0.46 ± 0.044***	0.27 ± 0.029***	0

Comment: The assays were carried out at a protein concentration of 41.17 mg/ml (for D. piger Vib-7) and 38.12 mg/ml (for Desulfomicrobium sp. Rod-9). Enzyme activity was determined after 10 min incubation. Statistical significance of the values M ± m, n = 5; ***P<0.001, compared to D. piger Vib-7 strain.