Fig. (2)
Growth of Enterobacteriaceae in the RWV. Panel A:
A representative growth curve for Enterobacteriaceae (Enterobacter cloacae
ATCC23355) in the RWV for the LSMMG and control conditions in LB media, 37 °C.
The RWV apparatuses were set-up and operated as shown in Fig. (1) and as
previously described [1, 3-5, 7]. At the indicated time points, aliquots were removed from the indicated
apparatuses, serial-diluted, and plated for CFU/ml counts in triplicate. This
experiment was also performed for Escherichia coli TOP10, Citrobacter
freudii ATCC8090, and Serratia marcsescens ATCC14041 with equivalent
results (data shown in panel B below).
Panel B: Cultures of the indicated bacteria were grown in the RWV
in the LSMMG and control conditions in LB media and samples processed as in panel A. For each time point, the ratio of CFU/ml
LSMMG to control was calculated. The results were obtained from at least two
independent cultures for each strain, and the average and standard deviation are
plotted. For E. coli, the data shown here is for strain TOP10. Strains
E. coli MG1655, E. coli DH5 α,
and E. coli AS11 displayed results equivalent to those presented here
(data not shown).