Contribution of Neuraminidase of Influenza Viruses to the Sensitivity to Sera Inhibitors and Reassortment Efficiency

Comparison Groups		t Distribution	p Value
Significance of Decreased Inhibitor Sensitivity in Reassortant Viruses (HAI titer)
WT viruses possessing inhibitor-resistant HA2 and inhibitor-resistant NA2 (HAI4 < 10)	6:2 WT/MDV reassortants possessing inhibitor-resistant HA2 and inhibitor-resistant NA2 (HAI < 10)	0	p > 0.99
WT viruses possessing inhibitor-sensitive HA3 and inhibitor- sensitive”NA3 (HAI = 2560-10240)	6:2 WT/MDV reassortants possessing inhibitor-sensitive HA3 and inhibitor-sensitive NA3 (HAI = 2560-10240)	0	p > 0.99
	7:1 reassortants possessing inhibitor-sensitive HA3 and inhibitor-resistant NA2 (HAI = 80-320)	11.09	p < 0.00001

¹Sensitivity to non-specific, heat-stable inhibitors in guinea pig serum.²Gene derived from a virus resistant to non-specific, heat-stable inhibitors in guinea pig serum.³Gene derived from a virus sensitive to non-specific, heat-stable inhibitors in guinea pig serum.⁴HAI: hemagglutination inhibition.