Contribution of Neuraminidase of Influenza Viruses to the Sensitivity to Sera Inhibitors and Reassortment Efficiency

Table .5: Sensitivity of influenza viruses to HAI by normal guinea pig sera.

Virus Tested		Origin of Gene			HAI Titer	Sensitivity to Inhibitors
Code	Strain Designation (Subtype)	Type/Subtype	HA	NA	HAI Titer	Sensitivity to Inhibitors
Influenza A Viruses
MDV¹	A-MDV	H2N2	MDV	MDV	< 10	Resistant⁴
WT²	A/California/07/04	H3N2	WT	WT	2560	Sensitive⁵
R1³	A/California/07/04 × MDV	H3N2	WT	MDV	320	Sensitive (<)⁷
R²	A/California/07/04 × MDV	H3N2	WT	WT	2560	Sensitive (=)⁷
WT	NIBRG-23	H5N1	WT	WT	5120	Sensitive
R³	NIBRG-23 × MDV	H5N2	WT	MDV	640	Sensitive (<)
R⁴	NIBRG-23 × MDV	H5N2	WT	MDV	640	Sensitive (<)
R⁵	NIBRG-23 × MDV	H5N2	WT	MDV	640	Sensitive (<)
WT	INDO/05	H5N1	WT	WT	10	Resistant
R⁶	INDO/05 × MDV	H5N2	WT	MDV	10	Resistant (=)
R⁷	INDO/05 × MDV	H5N2	WT	MDV	10	Resistant (=)
WT	VN1203	H5N1	WT	WT	< 10	Resistant
R⁸	VN1203 × MDV	H5N2	WT	MDV	< 10	Resistant (=)
R⁹	VN1203 × MDV	H5N2	WT	MDV	< 10	Resistant (=)
R10⁶	A/Vietnam/1203/2004 × MDV	H5N2	WT	WT	< 10	Resistant (=)
Influenza B Viruses
MDV	B-MDV	B	MDV	MDV	< 10	Resistant
WT	B/Harbin/07/94	B	WT	WT	10240	Sensitive
R11	B/Harbin/07/94 × MDV	B	WT	MDV	2560	Sensitive (<)
WT	B/Texas/26/08	B	WT	WT	< 10	Resistant
R12	B/Texas/26/08 × MDV	B	WT	MDV	< 10	Resistant (=)
R13	B/Texas/26/08 × MDV	B	WT	WT	< 10	Resistant (=)
WT	B/Wisconsin/1/10	B	WT	WT	5120	Sensitive
R14	B/Wisconsin/1/10 × MDV	B	WT	MDV	320	Sensitive (<)
R15	B/Wisconsin/1/10 × MDV	B	WT	WT	5120	Sensitive (=)
R16	B/Wisconsin/1/10 × MDV	B	WT	WT	5120	Sensitive (=)
WT	B/Bangladesh/1994/10	B	WT	WT	5120	Sensitive
R17	B/Bangladesh/1994/10 × MDV	B	WT	MDV	640	Sensitive (<)
WT	B/Texas/06/11	B	WT	WT	640	Sensitive
R18	B/Texas/06/11 × MDV	B	WT	MDV	80	Sensitive (<)
R19	B/Texas/06/11 × MDV	B	WT	WT	640	Sensitive (=)

¹MDV for LAIV: A/Leningrad/134/17/57 (H2N2) (A-MDV) or B/USSR/60/69 (B-MDV). ²WT virus or PR8-based virus inheriting HA and NA from WT virus. ³Reassortants of WT virus with MDV; the reassortants inherited HA from WT virus, and NA from WT virus (genomic composition 6:2) or MDV (genomic composition 7:1). ⁴Virus resistant to non-specific serum heat-stable inhibitors. ⁵Virus sensitive to non-specific serum heat-stable inhibitors. ⁶6:2 R10 reassortant virus generated by plasmid-based reverse genetics as described in [14]. ⁷Sensitivity of reassortant virus to non-specific serum heat-stable inhibitors compared to WT parental virus; p < 0.0001).