Table 1.: Effect due to different types and lots of peptone on colony characteristics and pigmentation of Ralstonia solanacearum NH-Av01 isolate on KM-TTC medium documented on day 2 following inoculum spotting.

A. Bacteriological Peptone (Hi-Media RM001; Enzymic hydrolysate of animal tissues)
Code Source code Manufacturing date/ date of opening Expiry date Condition  of peptone Colony visual characteristics (d2) Colony growth (A600) Pigmentation (A500 of supernatant)
A1 EMML / PT Feb. 2013/
Aug 2013
Feb. 2017 Powder Semi-fluidy, white; Light red centre 0.883 ±
0.221
0.004 (low)
A2 PHT / RS May 2006 / NA  May 2011 Slightly solidified Fluidy; Deep red with white outline 1.533 ±
0.012
0.061 (high)
A3 EN/ MSR July 2012 / just opened July 2016 Powder Fluidy; red with white outline 1.4097 ±
0.0770
0.063 (high)
A4 EMML/ PT Same as A1 but hydrated by loose capping Feb. 2017 Slightly hydrated More fluidy and darker color than A1 0.960 ±
0.0551
0.007 (low)
B. Type-1 peptone (Hi Media RM667; Mixed enzymic hydrolysate of lean meat tissues)
B1 EMML/ PT Oct 2013 July 2017 Powder Deep red semi-fluidy; white outline 1.594 ±
0.0231
0.120 (very high)
B2 TCL/ JBM Aug. 2010 Aug. 2015 Powder Fluidy; cream-white; light red centre 1.180 ±
0.0355
0.015 (Medium)
B3 SS/SK July 2010 July 2015 Powder Deep red; fluidy; white outline 1.482 ±
0.0417
0.098 (high)
B4 TL/MM NA May 2007* Slightly hydrated Fluidy; cream-white; Crimson red centre 1.331 ±
0.0110
0.074 (high)
C. Control. CPGA with A1 peptone; no TTC Feb. 2013/
Aug. 2013
Feb. 2017 Powder Fluidy cream white 1.238 ±
0.1067
-0.002 (none)

Date of conduct of the experiment: 17 Oct. 2013