Table 2.: Description of peptone belonging to different brands and lots (P1-P20) used in KM-TTC medium and the observations on colony characteristics of Ralstonia solanacearum isolate NH-Av01 recorded on day 2.

Media ID Details of the peptone lot used in KM-TTC medium Day 2 observations on colony growth on KM-TTC
Peptone brand and type Cat No & lot no †DoM/ DoE/ DoO as on product label Constitution/form Fluidity Diameter Color score White outline: prominence Colony growth (A600) Pigmentation (S/n‡ A500)
P1 Hi Media; Bacteriological RM001; 0000173838 06/13; 06/17; 10/13 Cream grainy powder Fluidy; red 10.6a 4 +++ 1.443c 0.080c,d
P2 Hi Media; Bacteriological RM001; 0000164122 02/ 13; 02/17; 08/13 Cream grainy powder Fluidy; pink 8.5d,e 3 ++ 1.236d 0.023e
P3 Hi Media; Bacteriological RM001; 0000164122 02/13; 02/17; 08/13 Cream powder; hydrated Fluidy; red 8.7d,e 3 ++ 1.293d 0.070c
P4 Hi Media; Bacteriological RM001; 0000148972 07/12; 07/16; 10/13 Cream grainy powder Fluidy; red 9.1c,d 4 +++ 1.660c 0.156a
P5 Hi Media; Bacteriological RM001; 000000873 01/10; 01/14; 10/13 Cream grainy powder Fluidy; maroon 9.8b,a 4 ++ 1.990a 0.166a
P6 Hi Media; Bacteriological RM001; 0000086521 05/06; 05/11€; NA Cream grainy powder Fluidy; maroon 10.0a,b 5 ++ 1.293d 0.096b
P7 Hi Media; Bacteriological RM001; 14213 NA; 06/06€; NA Cream grainy powder Fluidy; red 9.8b,c 4 ++ 2.026a,b 0.156a
P8 BD; BactoTM peptone 211677; 3007416 01/13; 01/17; 10/13 Fine powder Fluidy; maroon 8.1f 5 +++ 2.226a 0.113b
P9 Difco; Proteose peptone no.3 0122-01; 7500464 NA; NA; NA Yellow; crystalline Fluidy; maroon 9.8b,c 5 +++ 2.040a,b 0.086c
P10 Sigma/ Fluka; Microbiological Fluka 70172; BCBD0586 04/11; 04/15; NA Cream powder Fluidy; maroon 7.3g 5 ++ 1.896b 0.116b
P11 Biomatik Corporation; Bacteriological A8550; 063310323P BAC NA; NA; NA Cream powder Semi-fluidy; light red 8.5d,e 3 +++ 1.650c 0.023e,f
P12 FINAR Limited; Microbiological 11100;  87780 121 B11 02/11; NA; NA Cream yellow powder Non-fluidy; whitish 5.3i 1 - 0.541f -0.006f
P13 Sd-Fine; Bacteriological 54075; C112/0909/1808/61 NA; NA; NA Cream powder Semi-fluidy; light red 8.3e,f 2 +++ 0.930e 0.040e
P14 Hi-Media; Type 1-Bacteriological RM667; 000174965 07/13; 07/17; 10/13 Cream powder Fluidy; maroon 8.5d 5 + 2.150a 0.096b
P15 Hi-Media; Type 1-Bacteriological RM667; 000091730 08/10; 08/15; NA Cream powder Fluidy; deep maroon 6.9h 5 +++ 1.493c 0.070c
P16 Hi-Media; Type 1-Bacteriological RM667; 000119012 NA; 07/15; NA Cream powder Fluidy; deep red 7.5g,h 4 +++ 1.400c 0.050d,e
P17 Hi-Media; Veg peptone- Bacteriological RM001V; 000021713 NA; 08/12€; NA Cream powder Fluidy; intense maroon 8.6d 5 ++ 1.930b 0.100b,c
P18 Hi-Media; Mycological RM006; 000018316 NA; 06/12€;
NA 		Cream powder Fluidy; intense maroon 7.8f,g 5 ++ 2.120a,b 0.076c
P19 Hi-Media; General peptone CR 001; 4-2882 NA; NA€; NA (very old) Set; Caramel colour Fluidy; deep maroon 8.5d 5 + 2.046a,b 0.096b
CPGA Control; Same as P1, but without TTC RM001; 0000173838 06/13; 06/17; 10/13 Cream grainy powder Fluidy; cream white 8.6d 0 - 1.533c 0.063d
        Signifi
cance 		  P <0.01     P <0.01 P <0.01
        CD   0.724     0.264 0.0304

Notes:  † DoM, DoE and DoO; Date of manufacturing, expiry and opening, respectively; s/n‡, supernatant; NA, not available Values followed by the same letter in a column are not significantly different Color score 0, no pigmentation; 5, intense red-maroon; 1-4, intermediate Prominence of white colony outline; - not present; +, light, +++, very prominent P1-P7; Hi Media peptone, bacteriological (P1, Newly procured lot; P2, Batch where color issue was detected; P3, Same as P1 but  kept with loose capping; P4, One yr old but just opened): Enzymatic hydrolysate of animal tissues; P8: DIFCO BactoTM Peptone: Enzymatic digest of protein; P9: Proteos peptone; P10: tryptic digest of casein; P11-P13: Details not available; P14-P16: Hi Media Peptone Type 1, Bacteriological: Mixed enzymic hydrolysate of lean meat tissues; P17: Hi-Media; Veg peptone; enzymic hydrolysate of vegetable proteins; P18: Hi-Media; Mycological; Mixture of vegetable and plant peptones; P19: Peptone, certified, enzymic digestion of selected fresh meat. (€P6, P7, P17, P18, P19: Post-expiry date)