Table 3: Validation against Phenotype: agreement of MT PCR with phenotypic testing.

Phenotype Organism Total (n=126) MT PCR results
ESBL (Genotype) AmpC (Genotype) Carbapenemase (Genotype) Nil Detected
ESBL only 32 30
CTXM-1-18
CTXM-9-8  TEM-3
SHV-1
0 1
IMP-1
1
ESBL plus AmpC 15 13
CTXM-1-9
CTXM-9-2
TEM-2
1
DHA-1
0 1
ESBL plus carbapenemase 12 12
CTXM-1-7
CTXM-9-3
TEM-2
0 12
IMP-12
0
AmpC only 27 10
CTXM-1-5
CTXM-9-2
TEM-2
SHV-1
9
CMY-9
0 8
AmpC plus carbapenemase 3 0 3
CMY-3
3
IMP-3
0
Enterobacteriaceae producing carbapenemase  only 21 0 0 21
IMP-19
VIM-2
0
Non Fermenters producing Carbapenemase only 16 0 0 1
VIM-1
15
% Agreement MT PCR and phenotype Agreement value (%)
ESBL overall 59 93.2
AmpC overall 45 28.8
Carbapenemase 52 71.1