Table 4: Comparison of MT PCR to a second multiplex PCR for resistance detection.

MT PCR Result Second Multiplex PCR Concordant Second Multiplex PCR Discordant Examples of Difference Total
CTX-M-1 8 2 One CTX-M-9 in multiplex PCR, one nil detected 10
CTX-M-9 3 1 CTX-M-1 detected 4
TEM 0 4 TEM target not present in second multiplex PCR 4*
SHV 7 1 1 SHV not detected 8
CMY 2 0 No discrepancy 2
DHA 1 0 No discrepancy 1
IMP 10 1 1 IMP not detected by multiplex PCR 11
VIM 2 0 No discrepancy 2

* - excluded from analysis