Table 2: Detected T-RF sizes based on cyanobacterial T-RFLP profiling and in silico T-RF of the cyanobacterial 16S rRNA gene’s cloned sequence.

Methoda Sample/Clone Length (bp) HaeIII-T-RFs (bp) Identity (%) Closest sequence [Accession number]
T-RFLP KS2P   419.4    
  KS4P   419.3    
C.L. KS2-1 634 421 99 M. aeruginosa LMECYA 188 [EU078506]
  KS2-4 635 33 98 A. circinalis LMECYA 123 [EU078519]
  KS2-6 634 421 99 M. aeruginosa LMECYA 91B [EU078497]
  KS2-7, KS4-7, 18 634 421 99 M. aeruginosa LMECYA 110 [EU078499]
  KS2-10, KS4-8 634 421 99 M. aeruginosa LMECYA 151 [EU078502]
  KS2-12 634 421 99 M. aeruginosa LMECYA 29 [EU078489]
  KS4-1 634 421 99 M. novacekii BC18 [AB035551]
  KS4-6 634 421 99 M. aeruginosa NIES-843 [AP009552]
  KS4-14 634 421 100 M. aeruginosa LMECYA 110
  KS4-19 634 421 99 M. aeruginosa LMECYA 142 [EU078501]

a In T-RFLP profiling, the primers used were CYA106f and QCYA792r. For the clone library analysis, the primers used were CYA106f and CYA792r.C.L., clone library