Table 2: Kinetic parameters of the dissimilatory sulfate reduction by Desulfovibrio piger Vib-7 calculated from the approximation model of the logistic Verhulst functions.

Parameters Initial biomass (mg/ml) x0 µ0 t1 t2 te µmax
Bacterial
growth
0.12±0.011 0.2525±0.0230 0.0319±0.0029 21.9103±1.9918 62.8635±5.7149 42.3869±3.8534 0.0404±0.0037
0.25±0.024 0.6367±0.0579 0.0299±0.0027 7.1125±0.6466 52.5988±4.7817 29.8557±2.7142 0.0313±0.0028
0.5±0.048 0.7152±0.0650 0.0421±0.0038 8.7622±0.7966 26.4043±2.4004 17.5833±1.5985 0.0691±0.0063
1.0±0.096 1.7369±0.1579 0.0549±0.0050 3.4272±0.3116 20.9925±1.9084 12.2099±1.1100 0.0647±0.0059
Sulfate consumption 0.12±0.011 3.3733±0.3039 -0.0025±0.0002 65.6035±5.9102 27.1074±2.4421 46.3554±4.1762 -0.0025±0.0002
0.25±0.024 3.2361±0.2942 -0.0057±0.0005 49.7854±4.5259 15.0871±1.3716 32.4362±2.9487 -0.0057±0.0005
0.5±0.048 3.3734±0.3012 -0.0059±0.0005 28.2936±2.5262 12.0432±1.0753 20.1684±1.8008 -0.0059±0.0005
1.0±0.096 2.6653±0.2359 -0.0374±0.0033 15.7656±1.3952 -1.0225±0.0905 7.3715±0.6523 -0.0024±0.0002
Sulfide accumulation 0.12±0.011 0.1426±0.0130 0.0558±0.0051 27.0865±2.4624 72.1707±6.5610 49.6286±4.5117 0.0558±0.0051
0.25±0.024 0.2922±0.0266 0.0648±0.0059 12.5556±1.1414 49.3419±4.4856 30.9488±2.8135 0.0648±0.0059
0.5±0.048 0.8609±0.0783 0.0457±0.0042 -5.2212±0.4747 36.8641±3.3513 15.8214±1.4383 0.0457±0.0042
1.0±0.096 1.6985±0.1544 0.0231±0.0021 -29.3369±2.6670 24.3161±2.2106 2.5104±0.2282 0.0230±0.0021
Lactate consumption 0.12±0.011 16.5232±1.4886 -0.0025±0.0002 73.9492±6.6621 27.3945±2.4680 50.6718±4.5650 -0.0025±0.0002
0.25±0.024 15.9544±1.4504 -0.0051±0.0005 57.5076±5.2280 17.1392±1.5581 37.3234±3.3930 -0.0051±0.0005
0.5±0.048 15.6598±1.3982 -0.0101±0.0009 33.4879±2.9900 8.75958±0.7821 21.1237±1.8860 -0.0101±0.0009
1.0±0.096 16.3438±1.4464 -0.0103±0.0009 22.2445±1.9685 8.1212±0.7187 15.1829±1.3436 -0.0008±0.0001
Acetate accumulation 0.12±0.011 0.6582±0.0598 0.0598±0.0054 28.4587±2.5872 70.6445±6.4222 49.5516±4.5047 0.0598±0.0054
0.25±0.024 1.3403±0.1218 0.0964±0.0088 9.5692±0.8699 34.4944±3.1359 22.0318±2.0029 0.0964±0.0088
0.5±0.048 2.0966±0.1906 0.0799±0.0073 5.8945±0.5359 34.4062±3.1278 20.1503±1.8318 0.0799±0.0073
1.0±0.096 6.2578±0.5689 0.0408±0.0037 -12.3425±1.1220 27.9458±2.5405 7.8016±0.7092 0.0408±0.0037
Parameters Initial biomass (mg/ml) T1 (maximum acceleration of growth) Te (the point of curve inflection of growth acceleration) T2 (negative acceleration - deceleration of growth)
dx/dt1 µ1 dx/dte µe dx/dt2 µ2
Bacterial
growth
0.12±0.011 0.0233±0.0021 0.0403±0.0037 0.0349±0.0032 0.0289±0.0026 0.0233±0.0021 0.0128±0.0012
0.25±0.024 0.0248±0.0023 0.0313±0.0028 0.0372±0.0034 0.0242±0.0022 0.0248±0.0023 0.0109±0.0010
0.5±0.048 0.0797±0.0072 0.0678±0.0062 0.1195±0.0109 0.0569±0.0052 0.0797±0.0072 0.0263±0.0024
1.0±0.096 0.1335±0.0121 0.0545±0.0050 0.2003±0.0182 0.0546±0.0050 0.1121±0.0102 0.0256±0.0023
Sulfate consumption 0.12±0.011 -0.0362±0.0033 -0.0362±0.0033 -0.0543±0.0049 -0.0283±0.0025 -0.0362±0.0033 -0.0128±0.0012
0.25±0.024 -0.0427±0.0039 -0.0507±0.0046 -0.0639±0.0058 -0.0353±0.0032 -0.0427±0.0039 -0.0153±0.0014
0.5±0.048 -0.0941±0.0084 -0.1246±0.0111 -0.1411±0.0126 -0.0802±0.0072 -0.0941±0.0084 -0.0340±0.0030
1.0±0.096 -0.0913±0.0081 -0.1222±0.0108 -0.1369±0.0121 -0.0780±0.0069 -0.0913±0.0081 -0.0330±0.0029

Comments: t1, tе ,t2 are critical points on curves of the dissimilatory sulfate reduction by the strain Desulfovibrio piger Vib-7; х is the bacterial growth, sulfate and lactate consumption, sulfide and acetate accumulation; x0 is initial conditions of the strain at time t = 0; t is the time of the bacterial cultivation; te is the duration of the exponential phase; dx/dt is the absolute rate; µ0 is the specific rate at time t = 0; µmax is the maximum specific rate. Statistical significance of the values are means M±m, n = 5.