Fig. (3) Cleavage, detection and purification of mutacin(A) Silver staining of SDS−PAGE (15 %) of protein samples from the cleavage mixture before nickel affinity purification (lane 1), pure fraction containing His tagged proteins; His−sfGFP and sfGFP−TEV (lane 2) and the flow−through sample containing pure mutacin (lane 3). Gel filtration of injected nickel−purified flow−through sample from the cleavage reaction of sfGFP−mutacin in the presence of active (B) or of heat−inactivated form (C) of sfGFP−TEV. Gel filtration was achieved by injecting protein sample (Inject) into a Superdex200 column (10/30) at a flow rate of 1ml/min, and the fraction peak of pure mutacin is shown.