Fig. (3) Viral replication of wild-type, M184V-containing, D30N-containing, and M184V and D30N-containing viruses in vivo. Thy/liv implants were infected, separately (n=5 mice per group), with identical amounts of infectious units of each virus. Implants were analyzed following biopsy of infected tissue by quantitative PCR for HIV proviral DNA at 3, 5, and 7 weeks following infection. The amounts of proviral DNA are provided as copies of full length proviral DNA per 100,000 cells, as determined by the use of HIV and human β-globin specific primers and quantitative comparison to known controls. Mock infected controls were negative for HIV proviral DNA (not shown).