Fig. (2) Photomicrograph of ARPE19 cells without (A) and with (B,C,D) hydrogen peroxide treatment and stained with SA-BETA-GAL. ARPE-19 cells without hydrogen peroxide treatment (0 MCM) were cultured in conditioned media to full confluence and stained with SA-BETA-GAL for 24 hours, A. Results from cells treated with hydrogen peroxide are shown in B. (300MCM), C. (400 MCM and D. (800 MCM) (200 X magnification).