Table 1: Three multiplex PCR panels for the amplification of microsatellite loci in the Common Tern.
Multiplex Panels |
Locus |
Primer Sequence (5'–3') |
Dye |
Concentration [pmol/µL] |
Panel 1 |
K32 |
F: CATTGCACGAGTGTTAAGCTG |
FAM |
0.12 |
|
|
R: AAGGGTGCCTGTCCTTGTC |
|
|
|
MsSh22 |
F: GCCAGCTCTGCAATTCTACG |
HEX |
0.24 |
|
|
R: ACCCAGTAACCAGGCAGATG |
|
|
|
RBG18 |
F: AAAGGGCTGCTCATAGTACG |
HEX |
0.09 |
|
|
R: GTAGCATCATGTCTTCCCGC |
|
|
|
MsSh07 |
F: AGGCATTCTGAAATAGTGGGC |
NED |
0.09 |
|
|
R: AGGTCAGATATGTTCGTTGTGG |
|
|
|
MsSh03 |
F: GTTGGTGCTTGGAAGTAGGC |
NED |
0.20 |
|
|
R: AATGCCTGTCAGTTGGGTGG |
|
|
Panel 2 |
MsSh23 |
F: GCGCATGAATGAGAGACAATTG |
FAM |
0.11 |
|
|
R: TGTATCCATGGCTCAGCTACC |
|
|
|
MsSh18 |
F: AGAGCCAGGCACCCATATTC |
FAM |
0.23 |
|
|
R: CAGGTGGGTCGTCATGAGAG |
|
|
|
MsSh20 |
F: GTCCGTACAGAGGTTAACGC |
HEX |
0.12 |
|
|
R: ATGGTCACACTCGTCTCACC |
|
|
|
MsSh21 |
F: TGGTGTGCATGCATTTGTGG |
NED |
0.13 |
|
|
R: GGCATTAGTCAGGGTGTGTC |
|
|
|
MsSh31 |
F: AGAGAGAAGGAATTGGTGTAGC |
NED |
0.09 |
|
|
R: GGACCTGAGATCACCACAAG |
|
|
Panel 3 |
MsSh10 |
F: GCTGCGTATGTCCCAACTTG |
FAM |
0.12 |
|
|
R: CCAAATTGCTCCTCTGGCTG |
|
|
|
MsSh08 |
F: GTAGCCTCAGTACTGGTGGG |
FAM |
0.11 |
|
|
R: CCGTCTAAAGCCAGCACTTG |
|
|
|
MsSh09 |
F: GTGTCACCTGCCCAGAAATG |
HEX |
0.16 |
|
|
R: AGGGCATACATCCCTCATCC |
|
|
|
MsSh48 |
F: ATACACTGGCGGATGTTGTC |
HEX |
0.19 |
|
|
R: ACAAGATGACACAGAGCTGC |
|
|
|
MsSh37 |
F: AGCCAAACGACCTGAGTGAG |
NED |
0.14 |
|
|
R: GGAGAAGAGATGGTGTGTGC |
|
|
Each forward primer is labeled with one of three different fluorescent dyes.