Fig. (1) Method development with dye methylene blue on HCE-T monolayers grown on CS. Dye (0.1%) was used as a surrogate to determine experimental parameters on angle, number of needles, flow rates and elution zones on CS. Final parameters fixed on in vitro adhesion testing: declining channel at 11° and flow rate of ATF at 18 µL/min. A 35 µL sample of dye (equal later to one drop of ED) was placed between two needles, indicated herein by red dot. The picture was taken at approx. 1 min, demonstrating optimized elution zones. Within the total period of 5 min, most of the dye was eluted at the 1 min time point, confirming negligible binding.