Table 1: Primer Sequences, Size Products, and Conditions Used in RT-PCR Assays

Target Primer (5´- 3´) Size (bp) Annealing Temp/Cycles (ºC) Accession Number

18rS 1F-GGACAGGATTGACAGATTGATAG 101 60/12 R003278.1
R-CTCGTTCGTTATCGGAATTAAC

TNF-α F-GGCAGGTCTACTTTGGAGTCATTGC 307 59/40 NM013693.2
R-ACATTCGAGGCTCCAGTGAATTCGG

TGF-β F-GCCCTTCCTGCTCCTCAT 206 52/40 NM011577.1
R-TTGGCATGGTAGCCCTTG

IFN-γ F-AGCGGCTGACTGAACTCAGATTGTAG 247 52/45 NM008337.3
R-GTCACAGTTTTCAGCTGTATAGGG

1 F; Forward
R; Reverse.

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