Table 2: Diagnostic accuracy of different criteria published in the literature [¹⁴, ¹⁵, ²³-²⁵] utilizing different polymorphonuclear leukocytes (PML) staining techniques.

Study		Feldman [14]	Lonner [25]	Mirra [23]	Banit [15]	Athanasou [24]
Cut off		5 HPF all showing more than 5 PML	5 HPF all showing at least 10 PML	5 HPF one showing at least 5 PML	1 out of 5 HPFwith more than 10 PML	10 HPF having an average of more than 1 PML
HE-staining	Sensitivity	0.48	0.38	0.90	0.90	0.90
	Specificity	0.96	0.98	0.71	0.84	0.67
	PPV	0.83	0.89	0.54	0.70	0.51
	NPV	0.83	0.81	0.95	0.95	0.95
	Accuracy	0.83	0.82	0.76	0.86	0.73
MPOX-staining	Sensitivity	0.76	0.71	1.00	1.00	1.00
	Specificity	0.98	1.00	0.55	0.73	0.50
	PPV	0.94	1.00	0.46	0.58	0.43
	NPV	0.92	0.90	1.00	1.00	1.00
	Accuracy	0.92	0.92	0.68	0.81	0.64
NACE-staining	Sensitivity	0.67	0.62	0.95	0.95	0.95
	Specificity	1.00	1.00	0.54	0.68	0.52
	PPV	1.00	1.00	0.43	0.53	0.43
	NPV	0.89	0.87	0.97	0.97	0.97
	Accuracy	0.91	0.90	0.65	0.75	0.64