Table 3: Evaluation of overall diagnostic accuracy combining a highly sensitive initial screening criteria [¹⁵] followed by verification with a highly specific criteria [¹⁴].

Study		Banit [15]	Feldman [14]
Cut off		1 out of 5 HPFwith more than 10 PML	5 HPF all showing more than 5 PML
		1st step	2nd step (only positive results)
HE-staining	Sensitivity	0.48
	Specificity	0.96
	PPV	0.83
	NPV	0.83
	Accuracy	0.83
MPOX-staining	Sensitivity	0.76
	Specificity	1.00
	PPV	1.00
	NPV	0.92
	Accuracy	0.94
NACE-staining	Sensitivity	0.67
	Specificity	1.00
	PPV	1.00
	NPV	0.89
	Accuracy	0.91