Cryopreservation Effect on Proliferative and Chondrogenic Potential of Human Chondrocytes Isolated from Superficial and Deep Cartilage

Table 1: Primer Sequences, Conditions and Annealing Temperature of qRT-PCR Assays

Primersf	Sequence	Length	%GC	Annealing Temperature	Amplicon (bp)	Reference
CCND1 1F	5´ gac ctt cgt tgc cct ctg t 3´	19 mer	58	60°C	101	Universal ProbeLibrary
CCND 1R	5´ ggt tca ggc ctt gca ctg 3´	18 mer	61	60°C	101	Universal ProbeLibrary
SOX9 2F	5´gtacccgcacttgcacaac 3´	19	58	60°C	72	Universal ProbeLibrary
SOX9 2R	5´ tcgctctcgttcagaagtctc 3´	21	52	60°C	72	Universal ProbeLibrary
COL II 3F	5´ gtgtcagggccaggatgt 3´	18	61	60°C	116	Universal ProbeLibrary
COL II 3R	5´ tcccagtgtcacagacacagat 3´	22	50	60°C	116	Universal ProbeLibrary
HPRT 1F	5´ tga cct tga ttt att ttg cat acc 3´	24 mer	33	60°C	102	Universal ProbeLibrary
HPRT 1R	5´ cgagcaagacgttcagtcct 3´	20 mer	55	60°C	102	Universal ProbeLibrary

CCND1 = cyclin D1; SOX9 = SRY (sex determining region-Y)-box 9; COL II = type II collagen; HPRT = Hypoxanthine-guanine phosphoribosyltransferase.