Fig. (2) AHF Fed Animals Develop Mechanical Hypersensitivity. (A) Rearing events and (B) rearing duration were measured while animals were either on a smooth or rough surface. When animals were placed a rough surface, mice fed AHF diet reared significantly more often with increased rearing duration compared to animals fed control diet (p<0.05 by two-tailed t-test; Control n=7, AHF n=6). On a smooth surface, rearing events and duration were similar for both groups. (C) At chronic time points, week 7-12, paw withdrawal thresholds of AHF fed mice were significantly reduced compared to thresholds of control animals indicating hypersensitivity (p<0.05 by two-way ANOVA, Tukey post hoc test, n=6 per group). (D) Single low dose treatment with loperamide in week 12 significantly attenuated AHF-induced mechanical hypersensitivity within 1 h and mechanical sensitivity returned to pretreatment levels 3 hours post injection (p<0.05 by two-way ANOVA, Control n=4, AHF n=6). * p<0.05.