Table 2: Results of the host range analysis; specific observations in comparison to Acanthamoeba sp. strain LaHel, the “original” host amoebae [14, 15].

FLA (Acanthamoebae) Strain Specific Observations of the Amoeba - Pandoravirus Relationship; Proliferation Rate, in Comparison to the LaHel-KLaHel Findings
Acanthamoeba sp. LaHel Uptake of the virions immediatly after "co-cultivation"; after 2 days trophozoites were recognizable via light microscopy showing fulminante virus replication. One day later the trophozoites seem to be filled with virions only.
Acanthamoeba sp. Maha Longer adhesion times of the virions at the amoebal surface bevor uptake; proliferation in axenic and xenic cultures; 3 days after cocultivation viral particles inside the trophozoites. 7 days later trophozoites rounded up;
Acanthamoeba sp. MaTa Virusuptake in xenic culture immediatly after "co-cultivation" in axenic and xenic cultures; but limitate impact on the population; proliferation in axenic and xenic cultures;
Acanthamoeba sp. TaHa Fewer virus uptake compared to LaHel; immidiate virion uptake after "co-cultivation"; virion uptake in axenic cultures higher than in xenic cultures; encystation started from day 4 after cocultivation; trophozoites rounded up after 5 days; after 5 hours replication signs detectable; axenic similar
Acanthamoeba sp. Petz Similar to LaHel; immidiate virion uptake after "co-cultivation", higher encystation rate compared to LaHel; axenic similar; rounding up after three days
Acanthamoeba sp. Ulse Uptake starts 1,5 hours after "co-cultivation"; replication similar to Acanthamoeba sp. strain LaHel, the virions remained longer in the food vacuoles. I took three days until the virus particles could be detected within the cytoplasm of the amoebal trophozoites. A delayed developmental phase could be stated, compared to the development of Pandoravirus inopinatum in Acanthamoeba sp. strain LaHel. Apoptosios – like processes seemed to be involved, resulting in an increase in degraded or vacuolized trophozoites to prevent virus replication.
Acanthamoeba sp. Ubo Proliferation of Pandoravirus inopinatum within Acanthamoeba sp. Strain UBO only in the axenic medium; uptake similar to LaHel; uptake visible one day after cocultivation; virions visable; xenic: uptake could be no replication visable;
Acanthamoeba sp. Flobö Uptake of the virions visable after 1,5 hours after "co-cultivation"; the increased encystation rate seemed to be a result of the host - virus interaction; after 4-5 days incresed cell lysis; this strain was particularly suitable to document Pandoravirus virions within amoebal cysts.
Acanthamoeba sp. KLB Uptake similar; No replication signs in xenic medium; slow growing Acanthamoebae; axenisation not successful
Acanthamoeba sp. Smith Uptake immediatly; replication similar to KLaHel; three days after "Co-cultivation" many ruptured trophozoites;