FLA (Acanthamoebae) |
Strain |
Specific Observations of the Amoeba - Pandoravirus Relationship; Proliferation Rate, in Comparison to the LaHel-KLaHel Findings |
Acanthamoeba sp. |
LaHel |
Uptake of the virions immediatly after "co-cultivation"; after 2 days trophozoites were recognizable via light microscopy showing fulminante virus replication. One day later the trophozoites seem to be filled with virions only. |
Acanthamoeba sp. |
Maha |
Longer adhesion times of the virions at the amoebal surface bevor uptake; proliferation in axenic and xenic cultures; 3 days after cocultivation viral particles inside the trophozoites. 7 days later trophozoites rounded up; |
Acanthamoeba sp. |
MaTa |
Virusuptake in xenic culture immediatly after "co-cultivation" in axenic and xenic cultures; but limitate impact on the population; proliferation in axenic and xenic cultures; |
Acanthamoeba sp. |
TaHa |
Fewer virus uptake compared to LaHel; immidiate virion uptake after "co-cultivation"; virion uptake in axenic cultures higher than in xenic cultures; encystation started from day 4 after cocultivation; trophozoites rounded up after 5 days; after 5 hours replication signs detectable; axenic similar |
Acanthamoeba sp. |
Petz |
Similar to LaHel; immidiate virion uptake after "co-cultivation", higher encystation rate compared to LaHel; axenic similar; rounding up after three days |
Acanthamoeba sp. |
Ulse |
Uptake starts 1,5 hours after "co-cultivation"; replication similar to Acanthamoeba sp. strain LaHel, the virions remained longer in the food vacuoles. I took three days until the virus particles could be detected within the cytoplasm of the amoebal trophozoites. A delayed developmental phase could be stated, compared to the development of Pandoravirus inopinatum in Acanthamoeba sp. strain LaHel. Apoptosios – like processes seemed to be involved, resulting in an increase in degraded or vacuolized trophozoites to prevent virus replication. |
Acanthamoeba sp. |
Ubo |
Proliferation of Pandoravirus inopinatum within Acanthamoeba sp. Strain UBO only in the axenic medium; uptake similar to LaHel; uptake visible one day after cocultivation; virions visable; xenic: uptake could be no replication visable; |
Acanthamoeba sp. |
Flobö |
Uptake of the virions visable after 1,5 hours after "co-cultivation"; the increased encystation rate seemed to be a result of the host - virus interaction; after 4-5 days incresed cell lysis; this strain was particularly suitable to document Pandoravirus virions within amoebal cysts. |
Acanthamoeba sp. |
KLB |
Uptake similar; No replication signs in xenic medium; slow growing Acanthamoebae; axenisation not successful |
Acanthamoeba sp. |
Smith |
Uptake immediatly; replication similar to KLaHel; three days after "Co-cultivation" many ruptured trophozoites; |