Fig. (7) T. vaginalis trophozoites treated with Echinometra mathaei showed internalized flagella (IF) and externalized flagellea (F) (Fig. A). Its hydrogenosomes are of irregular shape and randomly distributed throughout the cytoplasm with altered matrix (Figs. A and B). A giant hydrogenosome with its altered matrix can be seen (black circle). Distortion of nucleus morphology (Fig. A) and plasma membrane (PM) (Fig. B) are shown. Several vacuoles within the cytoplasm have an autophagic appearance with internal membranes and a digested content that eventually fused with the parasite plasma membrane (PM). Lower cytoplasmic electron density with fragmented axostyle was observed (AX) (Fig. A).