Table 3: Antigen-induced proliferation of spleenocytes and DTH responses in mice and guinea-pigs, respectively, pre-immunized with rM. smegmatis-pDE22-PE35 and re-stimulated with the peptide pool and individual peptides of PE35.
| Peptides of PE35 used for re-stimulation of spleenocytes |
Animals immunized |
| Mice |
Guinea-pigs |
|
P/T1
|
P/T1
|
| Peptide pool of PE35 |
Not done |
4/4 |
| Individual peptides of PE35: |
| 1 |
1/5 |
2/4 |
| 2 |
1/5 |
3/4 |
| 3 |
3/5 |
2/4 |
| 4 |
2/5 |
3/4 |
| 5 |
2/5 |
3/4 |
| 6 |
0/5 |
0/4 |
1No. of animals showing positive responses / the no. of animals tested.