PROTOCOL |
- Sample type (tool, quantity, transport),
Treatment type (suspension-dilution),
Type of medium (total microflora, actinomycetes, fungi, algae, protozoa,
nitrogen cycle, carbon, sulfur) ,
Type of Inoculation, incubation and counts (CFU / MPN)
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Macroscopic and microscopic observation of isolated
Physiological and Biochemical test
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Extraction and cloning of DNA, with PCR Real Time, FISH, T-RFLP
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DGGE analysis
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Extraction and determination of the ATP
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Chemical analysis for nitrogen and organic carbon (TOC)
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Type of sampling classification and counting
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Device and methods used
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Composition and structure of the substrate (mineralogical and petro graphic analyses)
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