Table 2: Recoveries of BRs spiked in the plant at 25 ng/g (n=3).

Purification Method Castasterone Brassinolide
DCM 59.2 ± 4.1 80.4 ± 10.3
DCM+C18 42.9 ± 9.4 60.4 ± 3.6
ethyl acetate 25.4 ± 2.0 58.8 ± 5.1
ethyl acetate+C18 9.7 ± 2.7 43.3 ± 4.3
Hex+ethyl 8.8 ± 4.1 32.6 ± 2.5