Fig. (1) In vitro effects of Enalapril, Losartan and Valsartan after stimulation on PBMCs and their cytokines in RA. PBMCs from RA patients following stimulation with anti-CD3 and anti-CD28 mAB (SC) were treated with different conditions, Enalapril (E), Losartan (L) and Valsartan (V) at 100µM and Methylprednisolone (MP) at 100µM as standard drug. Cytokine concentrations of Th1/Th2/Th17 type cytokines were measured by ELISA in supernatants of PBMCs cultures from RA patients. A significant reduction in the IFN-γ and IL-22 concentration was detected in cultures of RA patients with Enalapril. Losartan reduced the concentration of IFN-γ; IL-6; IL-17F and IL-22 in PBMCs from RA patients. IL-2, IL-10 and IL-17A culture supernatants revealed no significant changes. Valsartan did not reduce any concentration in cytokines. (* = p <0.05; ** = p < 0.01; *** = p < 0.001 and **** = p< 0.0001).