Table 1: Primers used for quantitation of select gene expressions.
| Gene |
Froward Sequence (5′→3′) |
Reverse Sequence (5′→3’) |
Annealing Temperature (°C) |
| IL-6 |
CATTTGTGGTTGGGTCAGG |
AGTGAGGAACAAGCCAGAGC |
54 |
| IL-8 |
GAATGGGTTTGCTAGAATGTGATA |
CAGACTAGGGTTGCCAGATTTAAC |
52 |
| TNF-α |
CCTGCCCCAATCCCTTTATT |
CCCTAAGCCCCCAATTCTCT |
52 |
| IFN-γ |
TCGGTAACTGACTTGAATGTCCA |
TCCTTTTTCGCTTCCCTGTTTT |
54 |
|
CLEC4E (Mincle) |
CTACCGAGTCCGTGTCTACCA |
CATCCAAGTGCCAGCTAAGAG |
59 |
| BCL10 |
ATGGAGCCACGAACAACCTCT |
TCGTGCTGGATTCTCCTTCTG |
56 |
| RPLP0 |
GCTTCCTGGAGGGTGTCC |
GGACTCGTTTGTACCCGTTG |
55 |
| GAPDH |
GGTGGTCTCCTCTGACTTCAACA |
AGCTTGACAAAGTGGTCGTTGAG |
58 |
IL – interleukin, TNF – Tumour necrotic factor, IFN- interferon