Fig. (3). BAFF expression and secretion modulation by IFN-g stimulation on cultured epithelial cells. (A) BAFF mRNA expression was increased more than 80 fold by stimulation with IFN-g 10 ug/ml for 48 hours (Real-time PCR, primers and PCR condition as previously described, ref. 10). (B) BAFF concentration in the supernatant increased about 50 fold (from 20110.1 pg/ml to 10811357 pg/ml; measured by immunosorbent assay, R&D Systems Quantikine ELISA kit). The experiments were done two times in duplicate.