Fig. (2) Cytokine production by TNF-α treated DC. Unstimulated, LPS activated (1µg/ml for 4h) or TNF-α treated DC (500U/ml for 4h) were analyzed for the production of IL-12 and IL-10. (A) DC were stained for cell surface expression of CD11c and intracellular IL-12 and analyzed by flow cytometry. Quadrants were set using isotype control staining. Values indicate the percentage of cells in each quadrant. (B) Supernatants from the unstimulated, LPS activated or TNF-α treated DC were tested for IL-10 production by ELISA. Data shown are mean IL-10 production from duplicate samples and are representative of 2 separate experiments.