Fig. (5) Reduced CII specific IgG2a in sera obtained from mice injected with TNF-αtreated DC. Sera were collected at termination of the disease study, and levels of CII specific antibody determined by ELISA. (A) Total IgG. (B) IgG2a isotype. (C) IgG1 isotype. The antibody units reflect the relative amounts of CII specific antibody compared to a standard control serum (pooled sera from arthritic mice). This standard represented 100 units of antigen specific antibody. Levels of IgG2a and IgG1 have been normalised against amounts of total IgG detected in each sample. Values are the mean (n=8/group) ± SEM (* = p < 0.05 by Mann Whitney test).