Fig. (3) Western blot analysis of EBV gene expression in the TKS-1 cells. Expression of EBNA1 (top panel), EBNA2 (middle panel), and LMP1 (bottom panel) was examined in cell lysates of TKS-1, three subclones (1-3) of TKS-1, three (1-3) subclones of Akata, GL-1, CBL2, DI-1, and DI-5 by Western blotting as described in Materials and methods. Akata sublone 2 is an EBV- clone that has lost EBV DNA and therefore does not express EBNA1.