Fig. (1) A) Expression of the TIMP-4 gene in human synovial membranes from non-arthritic and osteoarthritic patients. The specific amplification of TIMP-4 and GAPDH fragments (arrows) along with the lambda Hind III or 100 bp ladder markers is shown. ‘No DNA’ control lane represents PCR reactions with primers but without cDNA. B) Middle panels show two graphic representations of semi-quantitative analysis of TIMP-4 RNA bands. *** p<0.001 Non-osteoarthritic (non-OA) versus osteoarthritic (OA). C) TIMP-4 RNA is expressed in non-OA (lane 1, 4, 5) and OA (lane 2, 3) synovial fibroblast lines from different patients.