Fig. (4) Fibrosis is associated with lipoatrophy. Skin biopsies demonstrating the association of dermal fibrosis with loss of the subjacent adipose tissue (adipose atrophy). A. SSc skin biopsy. Note dense dermal fibrosis and loss of subcutaneous fat. *, invasion of fibrotic tissue in adipose tissue. H&E stain, original magnification x 25. B. Skin biopsy from mouse with bleomycin-induced scleroderma. Note dense dermal fibrosis and absence of subcutaneous fat. Masson’s trichrome staining, original magnification x 100 (upper panel), 400 (lower panel).