Fig. (2) In vitro assay. (A) Effect of AEGH on lymphocyte cell
viability. Lymphocytes were incubated with AEGH at indicated
concentrations for 48 h. Each bar represents mean ± SEM.
Treatment with AEGH did not affect cell viability. (B) Effect of
AEGH on lymphocyte proliferation induced by ConA [5 μg/ml]
and treated with or without AEGH [80 μg/ml] for 48 h. Each bar
represents mean ± SEM (n=6). Cells incubated only with RPMI
medium were used as unstimulated controls. *P < 0.05 versus
ConA stimulated but non-treated cells, by Student’s unpaired t-test.
(C). Effect of AEGH on lymphocyte proliferation induced by LPS.
Lymphocytes were incubated with LPS [10 μg/ml] and treated with
or without AEGH [80 μg/ml] for 48 h. Each bars represents mean ±
SEM (n=6). Cells incubated only with RPMI medium was used as
unstimulated control. *P < 0.05 versus LPS stimulated but nontreated
cells, by Student’s unpaired t-test.