Table A1: Consistently negative ANA, positive subserologies case details.

Age Gender # Neg ANA Pos Subserologies Neg Subserologies Manifestations Diagnosis Outcome
34 F 2 SSA, RNP dsDNA, Sm, SSB leukopenia, anemia, Raynaud's, arthralgias SLE, FM placed on HCQ
64 F 1 Scl-70 cent, Jo-1 Raynaud's, arthralgias, NSIP concomitant stage III NSCLC; possible paraneoplastic process died due to ARDS and sepsis
19 F 2 SSA arthralgias, fatigue SLE,  FM placed on HCQ
38 F 6 dsDNA cent Raynaud's, alopecia, livedo possible SLE placed on HCQ, lost to follow up
31 F 2 Scl-70 SSA, SSB, Sm Raynaud's Raynaud's trial of HCQ not effective

ANA: antinuclear antibody; Pos: positive; Neg: negative; dsDNA: double stranded DNA; cent: centromere; SLE: systemic lupus erythematosus; FM: fibromyalgia; HCQ: hydroxychloroquine; NSIP: non-specific interstitial pneumonia; NSCLC: non-small cell lung cancer; ARDS: acute respiratory distress syndrome.