Fig. (3) Neovascularization (spout-outgrowth from human vascular endothelial cells (EC)-spheroids is increased in the presence of super natant (Sup Nat) obtained from asthmatic ASM cells (left panel). Images are representative for illustrating sprout outgrowth from ECspheroids incubated with supernatants of non-asthmatic and asthmatic ASM cells (as indicated). Lengths of sprouts are indicated as the calculated mean (±SD) of the longest 10 sprouts. * p < 0.05. Further details about sprouting and angiogenesis assays can be found in references 89 and 106.