Table 2: Microarray results displaying the most differentially expressed genes between feeder-cultured hESCs and MFG-hESCs (FC>10). A positive FC represents genes with increased expression in MFG-hESCs compared to feeder-cultured hESCs.

Gene Symbol Gene P-value FC
OPN / SPP1 Osteopontin / Secreted phosphoprotein 1 1.4E-04 54.03
TMEFF2 Transmembrane protein with EGF-like domains 2 1.9E-11 45.25
IGFBP7 Insulin-like growth factor binding protein 7 3.2E-05 39.40
NRK Nik related kinase 1.8E-03 30.79
CD58 / LFA-3 Lymphocyte function-associated antigen3 6.3E-12 25.99
CTHRC1 Collagen triple helix repeat containing 1 2.7E-04 25.40
ANXA1 Annexin A1 2.9E-05 23.34
EIF5B Eukaryotic translation initiation factor 5B 3.1E-14 20.79
CDC2 Cell division cycle 2 4.6E-09 19.40
ANKRD12 ankyrin repeat domain-containing protein 12 1.1E-12 18.24
COL12A1 Collagen, type XII, alpha 1 1.8E-08 14.93
FAM76B Family with sequence similarity 76, member B 5.6E-20 14.93
HPGD Hydroxyprostaglandin dehydrogenase 15-(NAD) 1.6E-03 14.15
NUDCD2 NudC domain containing 2 1.7E-19 13.40
DNAJA5 DnaJ homology subfamily A member 5 1.2E-04 13.30
TWIST1 Twist-related protein 1 2.5E-07 12.41
OR5T2 Olfactory receptor, family 5, subfamily T, member 2 1.6E-11 12.31
FANCB Fanconi anemia, complementation group B 6.4E-10 11.31
MRPS15 Mitochondrial ribosomal protein S15 3.4E-13 11.06
TXN Thioredoxin 1.9E-13 11.06
FKBP5 FK506 binding protein 5 4.3E-11 -17.55
PTPN11 Protein tyrosine phosphatase 6.2E-07 -17.68
ZMYND11 Zinc finger, MYND domain containing 11 1.6E-11 -18.10
TP53BP1 Tumor protein p53 binding protein, 1 5.4E-08 -18.38
PLK4 Polo-like kinase 4 (Drosophila) 3.4E-07 -19.70
LMAN1 Lectin, mannose-binding, 1 2.2E-05 -20.63
ERBB2 Human Epidermal growth factor Receptor 2 7.4E-16 -23.52
NUMA1 Nuclear mitotic apparatus protein 1 5.6E-15 -23.88
NUP188 Nucleoporin 188kDa 2.5E-10 -25.79
FOS Cellular proto-oncogene 4.1E-10 -28.73
EXOC4 Exocyst complex component 4 3.9E-08 -29.18
MLLT4 Myeloid/lymphoid or mixed-lineage leukemia 3.4E-15 -34.03
EWSR1 Ewing sarcoma breakpoint region 1 1.9E-07 -37.33
SFRS11 Splicing factor, arginine/serine-rich 11 2.3E-10 -37.33
DUSP6 Dual specificity phosphatase 6 1.7E-08 -43.88
TFRC/ CD71 Transferrin receptor (p90, CD71) 5.5E-09 -49.26
EGR1 Early growth response Protein 1 1.1E-12 -50.02
HEATR1 HEAT repeat containing 1 6.5E-06 -58.35
FOSB FBJ murine osteosarcoma viral oncogene homolog B 1.9E-08 -59.26
DHX9 DEAH (Asp-Glu-Ala-His) box polypeptide 9 9.8E-07 -60.18

Abbreviations: FC, fold change. hESCs, human embryonic stem cells. MFG-hESCs, matrix-free growth adapted human embryonic stem cells.