Table 3: Microarray results of differentially expressed genes involved in cellular attachment for feeder-cultured hESCs and MFG-hESCs. A positive FC represents genes with increased expression in MFG-hESCs compared to feeder-cultured hESCs.
| Gene Symbol |
Gene |
P-value |
FC |
| OPN |
Osteopontin |
1.0E-04 |
54.03 |
| ITGB6 |
Integrin beta 6 |
3.7E-02 |
19.10 |
| ITGB1 |
Integrin beta 1 |
1.0E-21 |
16.89 |
| CD47 |
CD47 |
7.6E-03 |
8.38 |
| COL4A3 |
Collagen IV alpha 3 |
1.0E-04 |
5.83 |
| ITGB5 |
Integrin beta 5 |
8.0E-04 |
4.52 |
| ITGB3 |
Integrin beta 3 |
1.0E-03 |
4.25 |
| THBS1 |
Thrombospondin |
6.0E-04 |
3.82 |
| CTSZ |
Cathepsin Z |
1.0E-21 |
3.68 |
| FBN1 |
Fibrillin 1 |
1.2E-02 |
3.43 |
| FN1 |
Fibronectin 1 |
1.3E-02 |
3.43 |
| MADCAM1 |
Mucosal addressin cell adhesion |
4.1E-03 |
2.81 |
| NAPSA |
Napsin A |
1.0E-21 |
2.39 |
| ITGA2B |
Integrin alpha 2 beta |
4.9E-02 |
2.33 |
| NID1 |
Nidogen 1 |
1.0E-21 |
2.26 |
| VWF |
Von Willebrand factor |
2.5E-02 |
2.13 |
| TNR |
Tenascin R |
4.0E-02 |
2.06 |
| ITGA9 |
Integrin alha 9 |
4.8E-02 |
2.05 |
| LAMA1 |
Laminin alpha 1 |
1.0E-21 |
-2.03 |
Abbreviations: FC, fold change. hESCs, human embryonic stem cells. MFG-hESCs, matrix-free growth adapted human embryonic stem cell.