Fig. (3) Phylogenetic relationships among Ureaplasma UreB and the homologues. (A) Neighbor-joining tree. The BLAST program was used to search the GenomeNet website for proteins homologous to Ureaplasma UreB with E-value < 10^-25. Ureaplasma UreA and 32 homologous proteins were multiple aligned using the CLUSTAL W program. Phylogenetic tree was reconstructed, based on the multiple-alignment with complete deletion of gap sites using the neighbor-joining method of MEGA software with 1000 bootstrap replicates. (B) Maximum likelihood tree. Phylogenetic tree was reconstructed using the maximum likelihood method of the PHYLIP program with 100 bootstrap replicates. The JTT model was used as the model of amino acid substitution. Number of times to jumble in the PROML program was 2.