Fig. (3) Effects of skin sensitizers on NRF2 transcriptional activity in MCF-7 cells under normal or high glucose conditions. MCF-7 cells were transfected with pAREx8-TATA and further incubated in various concentrations of eugenol (A, E), CA (B, F), DNCB (C, G), and isopropanol (D, H) under normal or high glucose conditions for 24 hours. The luciferase activity was determined, and the data were expressed as Relative Light Units (RLU: firefly luciferase activity divided by Renilla luciferase activity). All experiments were performed in triplicate, and values are indicated as mean ± S.D. Statistically significant differences are indicated by an asterisk (**p < 0.01, *p < 0.05 by Dunnett’s test).