Fig. (4) Effects of skin sensitizers on NRF2 transcriptional activity in BALB/3T3 clone A31 cells under normal or high glucose conditions. BALB/3T3 clone A31 cells were transfected with pAREx8-TATA, and further incubated in various concentrations of eugenol (A, E), CA (B, F), DNCB (C, G), and isopropanol (D, H) under normal or high glucose conditions for 24 hours. The luciferase activity was determined, and data were expressed as relative light units (RLU: firefly luciferase activity divided by Renilla luciferase activity). All experiments were performed in triplicate, and the values were indicated as mean ± S.D. Statistically significant differences are indicated by an asterisk (**p < 0.01, *p < 0.05 by Dunnett’s test).