Fig. (7) Effects of glucose on CA increased the mRNA level of HMOX1 in MCF-7 cells. (A): MCF-7 cells were incubated in the medium containing various concentrations (1.0 or 4.5 g/L) of D-glucose for 24 hours (Naive). (B): MCF-7 cells were incubated in the medium containing various concentrations (1.0 or 4.5 g/L) of D-glucose in the presence of 64 μM CA for 24 hours (CA treatment). The level of HMOX1 mRNA was analyzed by quantitative real-time PCR. GAPDH mRNA expression was used as an internal control. All experiments were performed in triplicate, and values are indicated as mean ± S.D. Statistically significant differences are indicated by an asterisk (**p<0.01 by Student’s t-test).