Fig. (10)
Immunocytochemistry. Detection of the podocyte
marker protein nephrin, the endothelial marker protein CD31 in podocyte isolates
and in total glomerular cell suspensions (glomerular cells) by immunocytochemistry
and in glomeruli in frozen sections of the renal cortex (glomerulus) by immunohistochemistry.
In podocyte isolates, most cells display a positive nephrin immunoreactivity
(red color), while only few cells show a positive CD31 staining (green color).
Nuclear stain DAPI (blue color). Bars = 5 μm.