Fig. (10) Immunocytochemistry. Detection of the podocyte marker protein nephrin, the endothelial marker protein CD31 in podocyte isolates and in total glomerular cell suspensions (glomerular cells) by  immunocytochemistry and in glomeruli in frozen sections of the renal cortex (glomerulus) by  immunohistochemistry. In podocyte isolates, most cells display a positive nephrin immunoreactivity (red color),  while only few cells show a positive CD31 staining (green color). Nuclear stain DAPI (blue color). Bars = 5 μm.