Fig. (11) Western-blot detection of the podocyte marker proteins WT1 and nephrin, the endothelial marker protein CD31, and of GAPDH (loading control). Per lane, 10 μg of protein, isolated from total renal  cortical tissue, from isolated glomeruli, and from isolated podocytes were loaded. Samples were pooled from  each two mice (n=6). Molecular weights of protein bands are indicated. Samples of isolated glomeruli and  isolated podocytes show intenser WT1 bands (arrows) and nephrin-bands, as compared to total renal cortical  tissue samples. In samples of isolated podocytes, the intensities of bands referring to CD31 are weaker, as compared to samples from isolated glomeruli and total kidney cortex.