Fig. (12)
RT-qPCR. Abundance of transcripts coding for the
podocyte markers nephrin (NPHS1) and Wilms tumor 1 (WT1), the endothelial marker
CD31, and the mesangial marker megsin (SERPINB7), relative to the abundance of
Gapdh-mRNA in samples of cortical kidney tissue, isolated glomeruli, and
isolated podocytes (n=4). Reflecting the marked enrichment of podocytes in
podocyte isolates and the higher proportion of podocytes in samples of isolated
glomeruli, as compared to total cortical kidney tissue samples, the relative Nphs1
and Wt1 mRNA abundances were the highest in podocyte isolates and the
lowest in the renal cortex, with that of isolated glomeruli lying in-between.
While the relative abundances of Nphs1 and Wt1 in samples of
isolated podocytes were significantly higher as compared to cortical kidney
tissue samples, the Nphs1 and Wt1 abundance increase in podocyte-
vs. and glomerulus isolates did not reach the level of statistical
significance. Corresponding to the higher proportion of endothelial and
mesangial cells in the glomerular capillary tuft as compared to total cortical
kidney tissue, and indicating a significant reduction of the proportion of
endothelial and mesangial glomerular cells in samples of isolated podocytes,
the relative Cd31 and Serpin7b mRNA abundances in samples of
isolated glomeruli were significantly higher than in total cortical kidney
tissue and in podocyte isolates. Although the relative Cd31 and
Serpin7b mRNA abundances in samples of isolated podocytes were slightly
higher than in samples of total cortical kidney tissue, these differences did
not reach the level of statistical significance. Data are means ±SEM.
Significant differences are indicated by asterisks. *: p<0.05; *: p<0.01;
ns: not significant.