Fig. (7) Effect of different concentrations, different storage conditions, and quality differences of NP suspensions on the efficiency
of NP-coating of isolated glomeruli. A: Isolated glomerulus after incubation
with a 3 mg/ml NP-in-CB-suspension. Only a thin and incomplete NP-layer is
present on the surface of isolated glomerulus (not suitable for subsequent
podocyte isolation). B, C: Isolated glomeruli after incubation
with a freshly prepared 10 mg/ml NP-in-CB-suspension (B, compare to Fig. 4A),
respectively with a 10 mg/ml NP-in-CB-suspension, that had been stored at 4-8°C
for three weeks (C, compare to Fig. 5Aa). All accessible outer glomerular
surfaces are coated with a homogenous, dense layer of NPs (suitable for
subsequent podocyte isolation). D: Isolated glomerulus after incubation
with a 30 mg/ml NP-in-CB-suspension. The NP coverage of the outer surfaces of
the isolated glomerulus displays an irregular thickness (not suitable for
subsequent podocyte isolation). E: Isolated glomerulus after incubation
with the NP-suspension shown in Fig. 4B. Multiple large NP-aggregates/clumps
are attached to the glomerulus, while large areas of the glomerular surface are
not covered by NPs (not suitable for subsequent podocyte isolation). Native,
unstained preparations. Bars = 50 μm.