Fig. (7) Effect of different concentrations, different storage conditions, and quality differences of NP suspensions on the efficiency of NP-coating of isolated glomeruli. A: Isolated glomerulus after incubation with  a 3 mg/ml NP-in-CB-suspension. Only a thin and incomplete NP-layer is present on the surface of isolated  glomerulus (not suitable for subsequent podocyte isolation). B, C: Isolated glomeruli after incubation with a  freshly prepared 10 mg/ml NP-in-CB-suspension (B, compare to Fig. 4A), respectively with a 10 mg/ml NP-in-CB-suspension, that had been stored at 4-8°C for three weeks (C, compare to Fig. 5Aa). All accessible outer glomerular surfaces are coated with a homogenous, dense layer of NPs (suitable for subsequent podocyte  isolation). D: Isolated glomerulus after incubation with a 30 mg/ml NP-in-CB-suspension. The NP coverage of  the outer surfaces of the isolated glomerulus displays an irregular thickness (not suitable for subsequent podocyte  isolation). E: Isolated glomerulus after incubation with the NP-suspension shown in Fig. 4B. Multiple large NP-aggregates/clumps are attached to the glomerulus, while large areas of the glomerular surface are not covered by  NPs (not suitable for subsequent podocyte isolation). Native, unstained preparations. Bars = 50 μm.