Fig. (9) Transmission electron microscopy (TEM) of podocyte isolation procedure steps. A, B: Isolated glomeruli. Peripheral podocytes (p), endothelial cells (e), and mesangial cells (m) are indicated. A: Isolated  glomerulus before coating with cationic colloidal silica-coated ferromagnetic nanoparticles (NPs). The inset to A  shows a detail enlargement of a podocyte on a peripheral glomerular capillary. B: Isolated glomerulus after  coating with NPs. The inset to B shows a detail enlargement of a peripheral glomerular capillary wall. C: Detail  enlargement of a peripheral podocyte in a glomerulus coated with NPs. In B and C, the glomerular  basement membrane (GBM) is indicated by asterisks. NPs attached to the podocyte surface, respectively the  surface of podocyte foot processes are marked by arrows. Arrowheads indicate the fenestrated endothelium.  Note the absence of NPs from the GBM and from endothelial and mesangial cell surfaces. D: Isolated podocyte  with NP’s on the cell surface (arrow). Note that some NPs will have detached from the cell surface during  processing of the sample for TEM. Bars = 1 μm.