Fig. (2) Quantification of HIV RNA Transcripts from the HIV-1 Genome during Cellular Infection. Transcriptional initiation and elongation were examined using quantitative RT-PCR that measures RNA transcripts that reach a relatively short distance and also a much larger distance from the start of transcription. Reverse transcription was performed using primers near the LTR (“HIV short-R”) and within the pol gene (“3kb-R”). The short and long transcripts were quantified using primer sets of equivalent efficiency that amplified transcripts of similar length (“HIVshort-F and HIVshort-R” for the short cDNA transcript and “3kb-F and 3kb-R” for the long cDNA transcript). Strategy adapted from Sune et al. [28].